High-dimensional and large-scale phenotyping of yeast mutants

Yoshikazu Ohya(Tokyo University of Science), Jun Sese(Tokyo University of Science), Masashi Yukawa(Tokyo University of Science), F. Sano(Tokyo University of Science), Yoichiro Nakatani(Tokyo University of Science), Taro Saito(Tokyo University of Science), Ayaka Saka(Tokyo University of Science), Tomoyuki Fukuda(Tokyo University of Science), Satoru Ishihara(Tokyo University of Science), Satomi Oka(Tokyo University of Science), Genjiro Suzuki(Tokyo University of Science), Machika Watanabe(Tokyo University of Science), Aiko Hirata(Tokyo University of Science), Miwaka Ohtani(Tokyo University of Science), Hiroshi Sawai(Tokyo University of Science), Nicolas Fraysse(Tokyo University of Science), Jean‐Paul Latgé(Tokyo University of Science), Jean François(Tokyo University of Science), Markus Aebi(Tokyo University of Science), Seiji Tanaka(Tokyo University of Science), Sachiko Muramatsu(Tokyo University of Science), Hiroyuki Araki(Tokyo University of Science), Kintake Sonoike(Tokyo University of Science), Satoru Nogami(Tokyo University of Science), Shinichi Morishita(Tokyo University of Science)
Proceedings of the National Academy of Sciences
December 19, 2005
10.1073/pnas.0509436102
Cited by 321Open Access
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Abstract

One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat morphological variations as quantitative traits. Deletion of nearly half of the yeast genes not essential for growth affects these morphological traits. Similar morphological phenotypes are caused by deletions of functionally related genes, enabling a functional assignment of a locus to a specific cellular pathway. The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome.

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