The Yeast Nuclear Pore Complex

Michael P. Rout(Rockefeller University), John D. Aitchison(University of Alberta), Adisetyantari Suprapto(Rockefeller University), Kelly Hjertaas(University of Alberta), Yingming Zhao(Rockefeller University), Brian T. Chait(Rockefeller University)
The Journal of Cell Biology
February 21, 2000
10.1083/jcb.148.4.635
Cited by 1,401Open Access
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Abstract

An understanding of how the nuclear pore complex (NPC) mediates nucleocytoplasmic exchange requires a comprehensive inventory of the molecular components of the NPC and a knowledge of how each component contributes to the overall structure of this large molecular translocation machine. Therefore, we have taken a comprehensive approach to classify all components of the yeast NPC (nucleoporins). This involved identifying all the proteins present in a highly enriched NPC fraction, determining which of these proteins were nucleoporins, and localizing each nucleoporin within the NPC. Using these data, we present a map of the molecular architecture of the yeast NPC and provide evidence for a Brownian affinity gating mechanism for nucleocytoplasmic transport.

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