Activities of Hydrogen Peroxide-Scavenging Enzymes in Germinating Wheat Seeds

Abstract

During imbibition and germination of wheat (Triticum aestivum) in the dark over 72 h, activities of the enzymes of the ascorbate (AsA)-dependent H 2 O 2 -scavenging pathway, AsA peroxidase, monodehydroascorbate (MDAsA) reductase, dehydroascorbate (DHAsA) reductase and glutathione (GSSG) reductase as well as superoxide dismutase (SOD), catalase and guaiacol peroxidase were determined both in whole grains and in isolated embryos and endosperm. With the exception of DHAsA reductase, activities of the other enzymes assayed increased in germinating seeds, especially during radicle emergence (between 24-48 h of imbibition)