Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants

Amy H.Y. Tong(University of Toronto), Marie Evangelista(Queen's University), Ainslie B. Parsons(University of Toronto), Hong Xu(University of Toronto), Gary D. Bader(Mount Sinai Hospital), Nicholas F. Page(McGill University), Mark D. Robinson(University of Toronto), Sasan Raghibizadeh(National Research Institute for Veterinary Virology and Microbiology of Russia), Christopher W.V. Hogue(Mount Sinai Hospital), Howard Bussey(McGill University), Brenda Andrews(University of Toronto), Mike Tyers(Mount Sinai Hospital), Charles Boone(University of Toronto)
Science
December 14, 2001
10.1126/science.1065810
Cited by 2,204

Abstract

In Saccharomyces cerevisiae, more than 80% of the approximately 6200 predicted genes are nonessential, implying that the genome is buffered from the phenotypic consequences of genetic perturbation. To evaluate function, we developed a method for systematic construction of double mutants, termed synthetic genetic array (SGA) analysis, in which a query mutation is crossed to an array of approximately 4700 deletion mutants. Inviable double-mutant meiotic progeny identify functional relationships between genes. SGA analysis of genes with roles in cytoskeletal organization (BNI1, ARP2, ARC40, BIM1), DNA synthesis and repair (SGS1, RAD27), or uncharacterized functions (BBC1, NBP2) generated a network of 291 interactions among 204 genes. Systematic application of this approach should produce a global map of gene function.

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