A novel microfluidic microplate as the next generation assay platform for enzyme linked immunoassays (ELISA)
Junhai Kai(Siloam Biosciences (United States)), Aniruddha Puntambekar(Siloam Biosciences (United States)), Nelson Santiago Vispo(Siloam Biosciences (United States)), Se Hwan Lee(Siloam Biosciences (United States)), David Sehy(Siloam Biosciences (United States)), Victor Moore(Siloam Biosciences (United States)), Jungyoup Han(Siloam Biosciences (United States)), Chong H. Ahn(Siloam Biosciences (United States))
Cited by 103
Abstract
In this work we introduce a novel microfluidic enzyme linked immunoassays (ELISA) microplate as the next generation assay platform for unparalleled assay performances. A combination of microfluidic technology with standard SBS-configured 96-well microplate architecture, in the form of microfluidic microplate technology, allows for the improvement of ELISA workflows, conservation of samples and reagents, improved reaction kinetics, and the ability to improve the sensitivity of the assay by multiple analyte loading. This paper presents the design and characterization of the microfluidic microplate, and its application in ELISA.
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