Imaging the Glycosylation State of Cell Surface Glycoproteins by Two‐Photon Fluorescence Lifetime Imaging Microscopy
Brian Belardi(Howard Hughes Medical Institute), Adam de la Zerda(Howard Hughes Medical Institute), David R. Spiciarich(Howard Hughes Medical Institute), Sophia L. Maund(Stanford University), Donna M. Peehl(Stanford University), Carolyn R. Bertozzi(Howard Hughes Medical Institute)
Cited by 101Open Access
Abstract
GLYCOPROTEINS IN FOCUS: Metabolic labeling of azido sugars combined with two-photon fluorescence lifetime imaging microscopy enables the visualization of specific glycoforms of endogenous proteins. This method can be utilized to detect glycosylated proteins in both cell culture and intact human tissue slices.
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