Two novel plasmid-mediated cefotaxime-hydrolyzing β-lactamases (CTX-M-5 and CTX-M-6) from<i>Salmonella typhimurium</i>

Maria Gazouli(Pasteur Hellenic Institute), Εva Tzelepi(Pasteur Hellenic Institute), Antonios Markogiannakis(National and Kapodistrian University of Athens), N.J. Legakis(National and Kapodistrian University of Athens), L. S. Tzouvelekis(National and Kapodistrian University of Athens)
FEMS Microbiology Letters
August 1, 1998
Cited by 65

Abstract

Two novel plasmid-mediated beta-lactamases (CTX-M-5 and CTX-M-6) produced by Salmonella typhimurium clinical strains were characterized. The enzymes exhibited a pI of 8.4, hydrolyzed oxyimino-beta-lactams and were susceptible to mechanism-based beta-lactamase inhibitors. The respective bla genes were cloned and sequenced. The deduced amino acid sequences showed a high degree of homology with those of the previously described plasmid class A CTX-M-type enzymes and appeared related to the chromosomal beta-lactamases of Klebsiella oxytoca.


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