Single-Molecule Approach to Enzymology

Abstract

Recent advances in single-molecule enzymology are reviewed. The theoretical underpinning of sincle-molecule enzymatic behaviors is discussed and exemplified by experiements and statistical analyses. In particular, the manifestations of the Michaelis-Menten mechanism, the kinetic scheme with sequential intermediates, and dynamic disorder in single molecule data are presented. A survey of current methods for single molecule enzymatic assays, especially those based on fluorescence detection, is presented as well.