Structural genomics of the <i>Thermotoga maritima</i> proteome implemented in a high-throughput structure determination pipeline

Scott A. Lesley(Joint Center for Structural Genomics), Peter Kühn(Joint Center for Structural Genomics), Adam Godzik(Joint Center for Structural Genomics), Ashley M. Deacon(Joint Center for Structural Genomics), Irimpan I. Mathews(Joint Center for Structural Genomics), Andreas Kreusch(Joint Center for Structural Genomics), Glen Spraggon(Joint Center for Structural Genomics), Heath E. Klock(Joint Center for Structural Genomics), Daniel McMullan(Joint Center for Structural Genomics), Tanya Shin(Joint Center for Structural Genomics), Juli Vincent(Joint Center for Structural Genomics), Alyssa Robb(Joint Center for Structural Genomics), Linda S. Brinen(Joint Center for Structural Genomics), Mitchell D. Miller(Joint Center for Structural Genomics), Timothy McPhillips(Joint Center for Structural Genomics), Mark A. Miller(Joint Center for Structural Genomics), Daniel Scheibe(Joint Center for Structural Genomics), Jaume M. Cánaves(Joint Center for Structural Genomics), Chittibabu Guda(Joint Center for Structural Genomics), Lukasz Jaroszewski(Joint Center for Structural Genomics), Thomas L. Selby(Joint Center for Structural Genomics), Marc‐André Elsliger(Joint Center for Structural Genomics), John Wooley(Joint Center for Structural Genomics), Susan S. Taylor(Takeda (Japan)), Keith O. Hodgson(Joint Center for Structural Genomics), Ian A. Wilson(Joint Center for Structural Genomics), Peter G. Schultz(Joint Center for Structural Genomics), Raymond C. Stevens(Joint Center for Structural Genomics)
Proceedings of the National Academy of Sciences
August 22, 2002
10.1073/pnas.142413399
Cited by 377Open Access
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Abstract

Structural genomics is emerging as a principal approach to define protein structure-function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to the proteome of the thermophilic bacterium Thermotoga maritima. By using this pipeline, we successfully cloned and attempted expression of 1,376 of the predicted 1,877 genes (73%) and have identified crystallization conditions for 432 proteins, comprising 23% of the T. maritima proteome. Representative structures from TM0423 glycerol dehydrogenase and TM0449 thymidylate synthase-complementing protein are presented as examples of final outputs from the pipeline.

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