Solid‐State Protein‐Structure Determination with Proton‐Detected Triple‐Resonance 3D Magic‐Angle‐Spinning NMR Spectroscopy

Abstract

Spinning a magical web: The combination of fast magic-angle spinning, isotopic dilution, and high magnetic field yields particularly well-resolved solid-state 1H NMR spectra, which are efficiently utilized to solve protein structure. New techniques are demonstrated, requiring only three days of data collection, to assign the proton signals and solve a high-resolution structure of microcrystalline GB1. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z702905_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.