Serine Proteases: Structure and Mechanism of Catalysis

Abstract

PERSPECTIVES AND SUMMARY .... 331 THE REACTION SEQUENCE AND INTERMEDIATES .... . .. . ..... 333 TRANSITION-STATE STABILIZATION .... .. . . . ... .. . 338 STRUCTURAL FEATURES PARTICIPATING IN CATALYSIS ........ 341 Polypeptide Binding on the Acyl-Group Side 342 Side-Chain Specificity Sites 344 Polypeptide Binding on the Leaving-Group Side 346 Tetrahedral Binding and the Oxyanion Binding Site 348 The Reactive Serine and the Charge Relay System . . . . . . . . . . . . . . . . . . . . . . 351