Targeting DNA Double-Strand Breaks with TAL Effector Nucleases
Michelle Christian(University of Minnesota), Tomáš Čermák(University of Minnesota), Erin Doyle(Iowa State University), Clarice Schmidt(Iowa State University), Feng Zhang(University of Minnesota), Aaron W. Hummel(Iowa State University), Adam J. Bogdanove(Iowa State University), Daniel F. Voytas(University of Minnesota)
Cited by 1,998Open Access
Abstract
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.
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