A BAC-Based Physical Map of the Major Autosomes of <i>Drosophila melanogaster</i>

Roger A. Hoskins(Lawrence Berkeley National Laboratory), Catherine R. Nelson(University of California, Berkeley), Benjamin P. Berman(University of California, Berkeley), Todd Laverty(University of California, Berkeley), Reed George(Lawrence Berkeley National Laboratory), L. D. Ciesiolka(Lawrence Berkeley National Laboratory), Mohammed Naeemuddin(Lawrence Berkeley National Laboratory), Andrew D. Arenson(Baylor College of Medicine), James Durbin(Baylor College of Medicine), Robert David(Baylor College of Medicine), Paul E. Tabor(Baylor College of Medicine), Michael R. Bailey(Baylor College of Medicine), Denise R. Deshazo(Baylor College of Medicine), Joseph J. Catanese(Roswell Park Comprehensive Cancer Center), Aaron Mammoser(Roswell Park Comprehensive Cancer Center), Kazutoyo Osoegawa(Roswell Park Comprehensive Cancer Center), † Pieter J. de(Roswell Park Comprehensive Cancer Center), Jong(Roswell Park Comprehensive Cancer Center), S Celniker(Lawrence Berkeley National Laboratory), Richard A. Gibbs(Baylor College of Medicine), Gerald M. Rubin(University of California, Berkeley), Steven E. Scherer(Baylor College of Medicine)
Science
March 24, 2000
10.1126/science.287.5461.2271
Cited by 146

Abstract

We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.

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