An o-Toluidine Method for Body-Fluid Glucose Determination

Abstract

This report described a simple, rapid, and versatile method for manual measurement of glucose in whole blood, serum, cerebrospinal fluid (CSF), and other body fluids, with results fully compatible with the then-widely used automated Hoffman procedure for body-fluid glucose: reduction of alkaline ferricyanide to ferrocyanide (1 ), adapted to the AutoAnalyzer. It also yielded results essentially specific for glucose, encompassed the full physiological range while discriminating readily between closely adjacent values, and was free from interference by most relevant substances.