Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodKenneth J. Livak, Thomas D. Schmittgen(Washington State University)MethodsDecember 1, 200110.1006/meth.2001.1262Cited by 180,613SaveCiteExport RISWatch citationsAbstractRelated PapersReal time quantitative PCR.Chris Heid, Junko Stevens, Kenneth J. Livak et al.|Genome Research|1996|6.2kThe Transcriptional Program in the Response of Human Fibroblasts to SerumVishwanath R. Iyer, Michael B. Eisen, Douglas T. Ross et al.|Science|1999|2kAn Overview of Real-Time Quantitative PCR: Applications to Quantify Cytokine Gene ExpressionAnnapaula Giulietti, Lut Overbergh, Dirk Valckx et al.|Methods|2001|1.4kDevelopment and Validation of Real-Time Quantitative Reverse Transcriptase–Polymerase Chain Reaction for Monitoring Gene Expression in Cardiac Myocytesin VitroJane Winer, Christopher Kwang S. Jung, Irene Shackel et al.|Analytical Biochemistry|1999|1.4kEffect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCRThomas D. Schmittgen, Brian A. Zakrajsek|Journal of Biochemical and Biophysical Methods|2000|1.2k