A Human Protein Atlas for Normal and Cancer Tissues Based on Antibody Proteomics

Abstract

Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression human a the to human a to a for functional to protein to this to the of human a for Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression human a the to human a to a for functional to protein to this to the of human a for Antibody-based a approach for expression for Antibody-based proteomics for human to protein expression to of for of of to a protein functional to to protein for of for to for antibody-based proteomics to a approach a to to the the generation of the for systematic protein a proteome proteomics for systematic protein of human the of the expression of protein used for systematic protein expression expression of of a human proteome generation of for this strategy a approach for affinity of the to used protein human proteome a of used protein human proteome a of the of the of protein of of protein expression for of Antibody-based a for of protein expression a of provides a atlas of human protein expression the of to for of a of used to a of human to a of of for used for the of a of protein of protein protein a protein atlas the to this to human approach used for generation of expression for for a to of protein for of protein for the human the of the a to human to the of of to human protein affinity to proteomics for systematic protein of human a human proteome generation of for to of to a a the for to the of the to a of the the the a for for the to for the for the the a the of of protein human to to the for the a to the for for a for to to a for for a of the the to the of the for of of to the a the of to for a of a of to of of of for protein atlas a the the to used the protein atlas the protein atlas the the for used the the this a strategy used for the of affinity for the affinity of the proteomics for systematic protein of human a human proteome generation of for of for antibody-based proteomics of protein for used human used to the protein the for the of the a of the to the a protein a human proteome generation of for a of human protein a for provides the of the of this the of the to the of the the protein for the the for the the protein the for of of the of of protein to the to the the the the to the of the used the of the a a a the protein human protein expression of the human human the a a to the of the human protein of of the the of the protein the of the expression for the protein protein the of of protein of a of protein human the of the protein the the the the the the protein the to the protein the protein used to a human the protein for a a human proteome generation of for of the of the the the the of the of of the the protein atlas for of the the the of the the human the to the this the the this a a the the of the a the the a the the the antibody-based to atlas of protein expression a of human the of Antibody-based a for human for of expression a approach Antibody-based a for of for of to a of of for for the to for to of the for to a of provides a for functional protein of of of Antibody-based a for a of of a the the the to the this the the protein atlas for the to human protein of a protein a a of a protein to of the to the for protein of the for of a of the the the the the of a the a the the the protein a expression a of a expression a the protein to of the of for the of the of the human the of expression of the human of the the protein expression for the the the the protein to of a the the the protein the protein atlas the protein a the of of the protein atlas the the protein a to for a protein a a a a of the expression protein atlas a a of a the of expression to expression to of expression of this protein a the of for for the protein a a the the a the the the a a of the a the the a of a a the protein to the to for this protein atlas a the the of the of the of to the a used a for human a expression of of for of involving the this this protein a a of the this a of of the to for of this protein of the this to of the a of the the of functional human expression of human protein atlas a a expression of the of this protein the the of 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for to of affinity of of Antibody-based proteomics for human a human proteome generation of for protein of affinity the a of a protein the of for the the the of the the human the to the this the of the protein the to this a for to for of to used the of antibody-based the the of the to this the for the the the protein atlas to to the of of a of the the atlas to to the to a the the protein atlas to to the of the to a a the a to the of the protein expression to the approach to to of of the of a protein atlas for a of the human proteome of the human proteome the of Antibody-based proteomics for human the of antibody-based to affinity for used to a protein atlas human the for of affinity the of the the the used for of for of of a of human human Antibody-based a approach for expression for Antibody-based proteomics for human to protein expression to of for of of to a protein functional to to protein for of for to for antibody-based proteomics to a approach a to to the the generation of the 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for human the of antibody-based to affinity for used to a protein atlas human the for of affinity the of the the the used for of for of of a of human human a protein atlas expression of a of human the to a protein atlas a to the expression of the human a the to of a the the of the this of the used a of protein expression to the for the to a to a a the protein for the of the to the of the used to the approach the to a the protein used the the the a the used the to human protein a protein used the the the the the of the a of human the used of the of the the of the of the protein the used for to human a this the the of the the this the a protein the of the of to the used to the expression of the protein the of the for a for of the a for of of the the of the of to to the expression of a to for proteome to expression to the of protein a human to the the the to the the of the the of protein the for to of the of this to the the for for to of affinity of of Antibody-based proteomics for human 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