Subdiffraction‐Resolution Fluorescence Imaging with Conventional Fluorescent Probes
Mike Heilemann(Bielefeld University), Sebastian van de Linde(Bielefeld University), Mark Schüttpelz(Bielefeld University), Robert Kasper(Bielefeld University), Britta Seefeldt(Bielefeld University), Anindita Mukherjee(Bielefeld University), Philip Tinnefeld, Markus Sauer(Bielefeld University)
Cited by 1,925
Abstract
Eagle eyes: dSTORM uses conventional photoswitchable fluorescent dyes that can be reversibly cycled between a fluorescent and a dark state by irradiation with light of different wavelengths (see picture). This elegant approach can visualize cellular structures with a resolution of approximately 20 nm, far beyond the diffraction limit of light, without the need of an activator molecule.
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