A physical sciences network characterization of non-tumorigenic and metastatic cells
David B. Agus(University of Southern California), Jenolyn F. Alexander(Houston Methodist), Wadih Arap(The University of Texas MD Anderson Cancer Center), Shashaanka Ashili(Arizona State University), Joseph E. Aslan(Oregon Health & Science University), Robert H. Austin(Princeton University), Vadim Backman(Northwestern University), Kelly Bethel(Scripps Clinic), Richard Bonneau(New York University), Wei‐Chiang Chen(Johns Hopkins University), Chira Chen‐Tanyolac(University of California, San Francisco), Nathan C. Choi(University of Southern California), Steven A. Curley(The University of Texas MD Anderson Cancer Center), Matthew Dallas(Johns Hopkins University), Dhwanil Damania(Northwestern University), Paul Davies(Arizona State University), Paolo Decuzzi(Houston Methodist), Laura E. Dickinson(Johns Hopkins University), Luis Estévez-Salmerón(University of California, San Francisco), Verónica Estrella(Moffitt Cancer Center), Mauro Ferrari(Houston Methodist), Claudia Fischbach(Cornell University), Jasmine Foo(University of Minnesota), Stephanie I. Fraley(Johns Hopkins University), Christian Frantz(University of California, San Francisco), Alexander Fuhrmann(Arizona State University), Philippe Gascard(University of California, San Francisco), Robert A. Gatenby(Moffitt Cancer Center), Yue Geng(Cornell University), Sharon Gerecht(Johns Hopkins University), Robert J. Gillies(Moffitt Cancer Center), Biana Godin(Houston Methodist), William M. Grady(University of Washington), Alex Greenfield(New York University), Courtney Hemphill(Arizona State University), Barbara L. Hempstead(Cornell University), Abigail Hielscher(Johns Hopkins University), W. Daniel Hillis(University of Southern California), Eric C. Holland(Memorial Sloan Kettering Cancer Center), Arig Ibrahim-Hashim(Moffitt Cancer Center), Tyler Jacks(Howard Hughes Medical Institute), Roger H. Johnson(Arizona State University), Ahyoung Joo(University of Southern California), Jonathan E. Katz(University of Southern California), Laimonas Kelbauskas(Arizona State University), Carl Kesselman(University of Southern California), Michael R. King(Cornell University), Κωνσταντίνος Κωνσταντόπουλος(Johns Hopkins University), Casey M. Kraning-Rush(Cornell University), Peter Kühn(Scripps Research Institute), Kevin S. Kung(University of Southern California), Brian J. Kwee(Cornell University), Johnathon N. Lakins(University of California, San Francisco), Guillaume Lambert(Princeton University), David Liao(University of California, San Francisco), Jonathan D. Licht(Northwestern University), Jan Liphardt(Lawrence Berkeley National Laboratory), Liyu Liu(Princeton University), Mark C. Lloyd(Moffitt Cancer Center), Anna Lyubimova(Massachusetts Institute of Technology), Parag Mallick(University of Southern California), John F. Marko(Northwestern University), Owen J. T. McCarty(Oregon Health & Science University), Deirdre R. Meldrum(Arizona State University), Franziska Michor(Dana-Farber Cancer Institute), Shannon M. Mumenthaler(University of Southern California), Vivek Nandakumar(Arizona State University), Thomas V. O’Halloran(Northwestern University), Steve Oh(University of California, San Francisco), Renata Pasqualini(The University of Texas MD Anderson Cancer Center), Matthew J. Paszek(University of California, San Francisco), Kevin G. Philips(Oregon Health & Science University), Christopher S. Poultney(Northwestern University), Kuldeepsinh Rana(Cornell University), Cynthia A. Reinhart‐King(Cornell University), Robert Ros(Arizona State University), Gregg L. Semenza(Johns Hopkins University), Patti Senechal(Arizona State University), Michael L. Shuler(Cornell University), Srimeenakshi Srinivasan(Houston Methodist), Jack R. Staunton(Arizona State University), Yolanda Stypula(Northwestern University), Hariharan Subramanian(Northwestern University), Thea D. Tlsty(University of California, San Francisco), Garth W. Tormoen(Oregon Health & Science University), Yiider Tseng(Johns Hopkins University), Alexander van Oudenaarden(Massachusetts Institute of Technology), Scott S. Verbridge(Cornell University), Jenny C. Wan(University of Southern California), Valerie M. Weaver(University of California, San Francisco), Jonathan Widom(Northwestern University), Christine Will(Northwestern University), Denis Wirtz(Johns Hopkins University), Jonathan W. Wojtkowiak(Moffitt Cancer Center), Pei‐Hsun Wu(Johns Hopkins University)
Cited by 181Open Access
Abstract
To investigate the transition from non-cancerous to metastatic from a physical sciences perspective, the Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of the non-tumorigenic MCF-10A and metastatic MDA-MB-231 breast epithelial cell lines, commonly used as models of cancer metastasis. Experiments were performed in 20 laboratories from 12 PS-OCs. Each laboratory was supplied with identical aliquots and common reagents and culture protocols. Analyses of these measurements revealed dramatic differences in their mechanics, migration, adhesion, oxygen response, and proteomic profiles. Model-based multi-omics approaches identified key differences between these cells' regulatory networks involved in morphology and survival. These results provide a multifaceted description of cellular parameters of two widely used cell lines and demonstrate the value of the PS-OC Network approach for integration of diverse experimental observations to elucidate the phenotypes associated with cancer metastasis.
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