In Vitro Cultivation of Human Tumors: Establishment of Cell Lines Derived From a Series of Solid Tumors2
Donald J. Giard, Stuart A. Aaronson(National Institutes of Health), George J. Todaro(National Institutes of Health), Paul Arnstein(California Department of Public Health), John H. Kersey(University of Minnesota Medical Center), Harvey Dosik(Jewish Hospital), Wade P. Parks(National Institutes of Health)
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Abstract
SUMMARY-A series of 200 human tumors were cultivated in vitro in an attempt to es-tablish cell lines. Lines were established with explant and trypsinization techniques, fr~m 13 tumors including ~arcinomas, sarcomas, mel-anomas, and brain tumors. All these lines in culture for over 1 year, exhibited marked refractility, multilayering, and criss-crossing and were morphologically distinct from nor-mal contact-inhibited human fibroblast or epithelial lines. They also formed colonies on IT!0nolay.e~s of n.ormal cells and grew with a high efficiency In soft agar. Preliminary re-sults!ndica~ed abnormal chromosomal pat-terns In all lines tested, and 8 of 9 cell lines
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