A Reducing and Oxidizing System Minimizes Photobleaching and Blinking of Fluorescent Dyes

Jan Vogelsang(Center for NanoScience), Robert Kasper(Bielefeld University), Christian Steinhauer(Center for NanoScience), Britta Person(Center for NanoScience), Mike Heilemann(Bielefeld University), Markus Sauer(Bielefeld University), Philip Tinnefeld(Center for NanoScience)
Angewandte Chemie International Edition
July 7, 2008
Cited by 628

Abstract

On the ROXS: Photobleaching represents one of the main limitations of modern fluorescence microscopy. A reducing and oxidizing system (ROXS) has been introduced that recovers triplet states as well as charge-separated states through electron-transfer reactions (see picture). Thus the blinking and photobleaching of fluorophores is strikingly reduced.


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