Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis
Milena Euler(University of Göttingen), Yongjie Wang(Shanghai Ocean University), Peter Otto(Friedrich-Loeffler-Institut), Herbert Tomaso(Friedrich-Loeffler-Institut), Raquel Escudero(Instituto de Salud Carlos III), Pedro Anda(Instituto de Salud Carlos III), Frank T. Hufert(University of Göttingen), Manfred Weidmann(University of Göttingen)
Cited by 157Open Access
Abstract
Several real-time PCR approaches to develop field detection for Francisella tularensis, the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant Tube Scanner device. The analytical sensitivity and specificity were tested using a plasmid standard and DNA extracts from infected rabbit tissues. The assay showed a performance comparable to real-time PCR but reduced the assay time to 10 min. The rapid RPA method has great application potential for field use or point-of-care diagnostics.
Related Papers
No related papers found
Powered by citation graph analysis