Identification of Non-Heme Diiron Proteins That Catalyze Triple Bond and Epoxy Group Formation
Michael A. Lee(Norwich Research Park), Marit Lenman(Norwich Research Park), Antoni Banaś(Norwich Research Park), Maureen Bafor(Norwich Research Park), Surinder Singh(Norwich Research Park), Michael Schweizer(Norwich Research Park), Ralf Nilsson(Norwich Research Park), Conny Liljenberg(Norwich Research Park), Anders Dahlqvist(Norwich Research Park), Per-Olov Gummeson(Norwich Research Park), Staffan Sjödahl(Norwich Research Park), Allan Green(Norwich Research Park), Sten Stymne(Norwich Research Park)
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Abstract
Acetylenic bonds are present in more than 600 naturally occurring compounds. Plant enzymes that catalyze the formation of the Delta12 acetylenic bond in 9-octadecen-12-ynoic acid and the Delta12 epoxy group in 12,13-epoxy-9-octadecenoic acid were characterized, and two genes, similar in sequence, were cloned. When these complementary DNAs were expressed in Arabidopsis thaliana, the content of acetylenic or epoxidated fatty acids in the seeds increased from 0 to 25 or 15 percent, respectively. Both enzymes have characteristics similar to the membrane proteins containing non-heme iron that have histidine-rich motifs.
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