Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range
Sidney Udenfriend(La Roche College), Stanley J. Stein(La Roche College), Peter Böhlen(La Roche College), Wallace Dairman(La Roche College), W. Leimgruber(La Roche College), Manfred Weigele(La Roche College)
Cited by 2,655
Abstract
Fluorescamine is a new reagent for the detection of primary amines in the picomole range. Its reaction with amines is almost instantaneous at room temperature in aqueous media. The products are highly fluorescent, whereas the reagent and its degradation products are nonfluorescent. Applications are discussed.
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