Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences
Axel Ullrich(University of California, San Francisco), John Shine(University of California, San Francisco), John M. Chirgwin(University of California, San Francisco), Raymond Pictet(University of California, San Francisco), Edmund Tischer(University of California, San Francisco), William J. Rutter(University of California, San Francisco), Howard M. Goodman(University of California, San Francisco)
Cited by 1,254
Abstract
Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3' terminal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II.