Wild-type p53 binds to the TATA-binding protein and represses transcription.
Edward Seto(Howard Hughes Medical Institute), Anny Usheva(Howard Hughes Medical Institute), Gerard P. Zambetti(Howard Hughes Medical Institute), Jamil Momand(Howard Hughes Medical Institute), Nobuo Horikoshi(Howard Hughes Medical Institute), Roberto Weinmann(The Wistar Institute), Arnold J. Levine(Howard Hughes Medical Institute), Thomas Shenk(Howard Hughes Medical Institute)
Cited by 520Open Access
Abstract
p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.
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