A Golden Gate Modular Cloning Toolbox for Plants
Carola Engler, Mark Youles(Sainsbury Laboratory), Ramona Gruetzner(Leibniz Institute of Plant Biochemistry), Tim-Martin Ehnert, Stefan Werner, Jonathan D. G. Jones(Norwich Research Park), Nicola J. Patron(Sainsbury Laboratory), Sylvestre Marillonnet(Leibniz Institute of Plant Biochemistry)
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Abstract
Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation. Parts include promoters, untranslated sequences, reporters, antigenic tags, localization signals, selectable markers, and terminators. The comparative performance of parts in the model plant Nicotiana benthamiana is discussed.