Semiconductor Nanocrystals as Fluorescent Biological Labels
Marcel P. Bruchez(Lawrence Berkeley National Laboratory), Mario M. Moronne(Lawrence Berkeley National Laboratory), Peter Gin(Lawrence Berkeley National Laboratory), Shimon Weiss(Lawrence Berkeley National Laboratory), A. Paul Alivisatos(Lawrence Berkeley National Laboratory)
Cited by 8,582
Abstract
Semiconductor nanocrystals were prepared for use as fluorescent probes in biological staining and diagnostics. Compared with conventional fluorophores, the nanocrystals have a narrow, tunable, symmetric emission spectrum and are photochemically stable. The advantages of the broad, continuous excitation spectrum were demonstrated in a dual-emission, single-excitation labeling experiment on mouse fibroblasts. These nanocrystal probes are thus complementary and in some cases may be superior to existing fluorophores.
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