Heparin Affinity: Purification of a Tumor-Derived Capillary Endothelial Cell Growth Factor
Yuen Shing(Boston Children's Hospital), Judah Folkman(Boston Children's Hospital), Robert Sullivan(Boston Children's Hospital), Catherine Butterfield(Boston Children's Hospital), Joanne F. Murray(Boston Children's Hospital), Michael Klagsbrun(Boston Children's Hospital)
Cited by 936
Abstract
A tumor-derived growth factor that stimulates the proliferation of capillary endothelial cells has a very strong affinity for heparin. This heparin affinity makes it possible to purify the growth factor to a single-band preparation in a rapid two-step procedure. The purified growth factor is a cationic polypeptide, has a molecular weight of about 18,000, and stimulates capillary endothelial cell proliferation at a concentration of about 1 nanogram per milliliter.
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