Novel Stem/Progenitor Cell Population from Murine Tracheal Submucosal Gland Ducts with Multipotent Regenerative Potential

Ahmed E. Hegab(University of California, Los Angeles), Vi Luan Ha(University of California, Los Angeles), Jennifer L. Gilbert(University of California, Los Angeles), Kelvin Xi Zhang(Howard Hughes Medical Institute), Stephen P. Malkoski(Denver Health Medical Center), Andy T. Chon(University of California, Los Angeles), Daphne O. Darmawan(University of California, Los Angeles), Bharti Bisht(University of California, Los Angeles), Aik T. Ooi(University of California, Los Angeles), Matteo Pellegrini(University of California, Los Angeles), Derek W. Nickerson(University of California, Los Angeles), Brigitte N. Gomperts(University of California, Los Angeles)
Stem Cells
June 30, 2011
10.1002/stem.680
Cited by 134

Abstract

The airway epithelium is in direct contact with the environment and therefore constantly at risk for injury. Basal cells (BCs) have been found to repair the surface epithelium (SE), but the contribution of other stem cell populations to airway epithelial repair has not been identified. We demonstrated that airway submucosal gland (SMG) duct cells, in addition to BCs, survived severe hypoxic-ischemic injury. We developed a method to isolate duct cells from the airway. In vitro and in vivo models were used to compare the self-renewal and differentiation potential of duct cells and BCs. We found that only duct cells were capable of regenerating SMG tubules and ducts, as well as the SE overlying the SMGs. SMG duct cells are therefore a multipotent stem cell for airway epithelial repair This is of importance to the field of lung regeneration as determining the repairing cell populations could lead to the identification of novel therapeutic targets and cell-based therapies for patients with airway diseases.

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