Complete genome sequence and comparative analysis of the metabolically versatile <i>Pseudomonas putida</i> KT2440

William Nelson(J. Craig Venter Institute), Christian Weinel(Medizinische Hochschule Hannover), Ian T. Paulsen(J. Craig Venter Institute), Robert J. Dodson(J. Craig Venter Institute), H. Hilbert(Qiagen (Spain)), Vítor A. P. Martins dos Santos(Helmholtz Centre for Infection Research), Derrick E. Fouts(J. Craig Venter Institute), Steven R. Gill(J. Craig Venter Institute), Mihai Pop(J. Craig Venter Institute), Megan E. Holmes(J. Craig Venter Institute), Lauren Brinkac(J. Craig Venter Institute), Maureen J. Beanan(J. Craig Venter Institute), Robert T. DeBoy(J. Craig Venter Institute), S. Daugherty(J. Craig Venter Institute), James F. Kolonay(J. Craig Venter Institute), Ramana Madupu(J. Craig Venter Institute), William Nelson(J. Craig Venter Institute), Owen White(J. Craig Venter Institute), J. Peterson(J. Craig Venter Institute), Hoda Khouri(J. Craig Venter Institute), Ioana R. Hance(J. Craig Venter Institute), P. Chris Lee(J. Craig Venter Institute), Erik Holtzapple(J. Craig Venter Institute), David J. Scanlan(J. Craig Venter Institute), Khiem Tran(J. Craig Venter Institute), A. Moazzez(J. Craig Venter Institute), Teresa R. Utterback(J. Craig Venter Institute), Michael Rizzo(J. Craig Venter Institute), Kyelim Lee(J. Craig Venter Institute), D. Kosack(J. Craig Venter Institute), D. Moestl(Qiagen (Spain)), Holger Wedler(Qiagen (Spain)), Jürgen Lauber(Qiagen (Spain)), D. Stjepandic(German Cancer Research Center), Jörg D. Hoheisel(German Cancer Research Center), M. Straetz(Helmholtz Centre for Infection Research), Sabina Heim(Helmholtz Centre for Infection Research), Claudia Kiewitz(Medizinische Hochschule Hannover), Jonathan A. Eisen(J. Craig Venter Institute), Kenneth N. Timmis(Helmholtz Centre for Infection Research), Andreas Düsterhöft(Qiagen (Spain)), Burkhard Tümmler(Medizinische Hochschule Hannover), Claire M. Fraser(J. Craig Venter Institute)
Environmental Microbiology
December 1, 2002
10.1046/j.1462-2920.2002.00366.x
Cited by 1,351

Abstract

Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as a biosafety host for the cloning of foreign genes. The bacterium also has considerable potential for biotechnological applications. Sequence analysis of the 6.18 Mb genome of strain KT2440 reveals diverse transport and metabolic systems. Although there is a high level of genome conservation with the pathogenic Pseudomonad Pseudomonas aeruginosa (85% of the predicted coding regions are shared), key virulence factors including exotoxin A and type III secretion systems are absent. Analysis of the genome gives insight into the non-pathogenic nature of P. putida and points to potential new applications in agriculture, biocatalysis, bioremediation and bioplastic production.

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