In Situ Visualization of DNA Double-Strand Break Repair in Human Fibroblasts
Benjamin E. Nelms(University of Wisconsin–Madison), Richard S. Maser(University of Wisconsin–Madison), James F. MacKay(University of Wisconsin–Madison), M. G. Lagally(University of Wisconsin–Madison), John H.J. Petrini(University of Wisconsin–Madison)
Cited by 497
Abstract
A method was developed to examine DNA repair within the intact cell. Ultrasoft x-rays were used to induce DNA double-strand breaks (DSBs) in defined subnuclear volumes of human fibroblasts and DNA repair was visualized at those sites. The DSBs remained in a fixed position during the initial stages of DNA repair, and the DSB repair protein hMre11 migrated to the sites of damage within 30 minutes. In contrast, hRad51, a human RecA homolog, did not localize at sites of DNA damage, a finding consistent with the distinct roles of these proteins in DNA repair.
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