Sequence Finishing and Mapping of <i>Drosophila melanogaster</i> Heterochromatin

Roger A. Hoskins(Lawrence Berkeley National Laboratory), Joseph W. Carlson(Lawrence Berkeley National Laboratory), Cameron Kennedy(Lawrence Berkeley National Laboratory), Daniel Estrada Acevedo(Lawrence Berkeley National Laboratory), Martha Evans-Holm(Lawrence Berkeley National Laboratory), Erwin Frise(Lawrence Berkeley National Laboratory), Kenneth H. Wan(Lawrence Berkeley National Laboratory), Soo Hyung Park(Lawrence Berkeley National Laboratory), María Méndez-Lago(Lawrence Berkeley National Laboratory), Fabrizio Rossi(Lawrence Berkeley National Laboratory), Alfredo Villasanté(Lawrence Berkeley National Laboratory), Patrizio Dimitri(Lawrence Berkeley National Laboratory), Gary H. Karpen(Lawrence Berkeley National Laboratory), S Celniker(Lawrence Berkeley National Laboratory)
Science
June 14, 2007
10.1126/science.1139816
Cited by 341

Abstract

Genome sequences for most metazoans and plants are incomplete because of the presence of repeated DNA in the heterochromatin. The heterochromatic regions of Drosophila melanogaster contain 20 million bases (Mb) of sequence amenable to mapping, sequence assembly, and finishing. We describe the generation of 15 Mb of finished or improved heterochromatic sequence with the use of available clone resources and assembly methods. We also constructed a bacterial artificial chromosome–based physical map that spans 13 Mb of the pericentromeric heterochromatin and a cytogenetic map that positions 11 Mb in specific chromosomal locations. We have approached a complete assembly and mapping of the nonsatellite component of Drosophila heterochromatin. The strategy we describe is also applicable to generating substantially more information about heterochromatin in other species, including humans.

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