Genes Required for Mitotic Spindle Assembly in <i>Drosophila</i> S2 Cells
Gohta Goshima(Marine Biological Laboratory), Roy Wollman(Marine Biological Laboratory), Sarah S. Goodwin(Marine Biological Laboratory), Nan Zhang(Marine Biological Laboratory), Jonathan M. Scholey(Marine Biological Laboratory), Ronald D. Vale(Marine Biological Laboratory), Nico Stuurman(Marine Biological Laboratory)
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Abstract
The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.
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