Green Fluorescent Protein as a Marker for Gene Expression
Martin Chalfie(Columbia University), Yuan Tu(Columbia University), Ghia Euskirchen(Columbia University), William W. Ward(Rutgers, The State University of New Jersey), Douglas C. Prasher(Woods Hole Oceanographic Institution)
Cited by 6,754
Abstract
A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.
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