A general method for the purification of restriction enzymes

Patricia J. Greene(University of California, San Francisco), Herbert L. Heyneker(University of California, San Francisco), Francisco Bolívar(University of California, San Francisco), Raymond L. Rodriguez(University of California, San Francisco), Mary C. Betlach(University of California, San Francisco), Alejandra A. Covarrubias(University of California, San Francisco), Keith Backman(University of California, San Francisco), David J. Russel(University of California, San Francisco), Robert C. Tait(University of California, San Francisco), Herbert W. Boyer(University of California, San Francisco)
Nucleic Acids Research
January 1, 1978
10.1093/nar/5.7.2373
Cited by 314Open Access
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Abstract

An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.

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