Methods to Study GPI Anchoring of Proteins

Abstract

Dropping anchor: Glycosylphosphatidylinositol (GPI) membrane-anchored proteins noncovalently associate with the plasma membrane and can have an impact on oncogenesis and some infectious diseases. The GPI anchor biosynthetic machinery and GPI-T, the transamidase that attaches them to proteins, are complicated, membrane-associated enzymes that are only beginning to be understood.