Structure of the 70 <i>S</i> Ribosome Complexed with mRNA and tRNA
M. Selmer(MRC Laboratory of Molecular Biology), C.M. Dunham(MRC Laboratory of Molecular Biology), F.V. Murphy(MRC Laboratory of Molecular Biology), Albert Weixlbaumer(MRC Laboratory of Molecular Biology), Sabine Petry(MRC Laboratory of Molecular Biology), Ann C. Kelley(MRC Laboratory of Molecular Biology), John R. Weir(MRC Laboratory of Molecular Biology), V. Ramakrishnan(MRC Laboratory of Molecular Biology)
Cited by 1,289
Abstract
The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.
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