Preparing Protein Microarrays by Soft-Landing of Mass-Selected Ions
Zheng Ouyang(Purdue University West Lafayette), Zoltán Takáts(Purdue University West Lafayette), Thomas A. Blake(Purdue University West Lafayette), Bogdan Gologan(Purdue University West Lafayette), Andy J. Guymon(Purdue University West Lafayette), Justin M. Wiseman(Purdue University West Lafayette), Justin C. Oliver(Purdue University West Lafayette), V. Jo Davisson(Purdue University West Lafayette), R. Graham Cooks(Purdue University West Lafayette)
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Abstract
Intact, multiply protonated proteins of particular mass and charge were selected from ionized protein mixtures and gently landed at different positions on a surface to form a microarray. An array of cytochrome c, lysozyme, insulin, and apomyoglobin was generated, and the deposited proteins showed electrospray ionization mass spectra that matched those of the authentic compounds. Deposited lysozyme and trypsin retained their biological activity. Multiply charged ions of protein kinase A catalytic subunit and hexokinase were also soft-landed into glycerol-based liquid surfaces. These soft-landed kinases phosphorylated LRRASLG oligopeptide and D-fructose, respectively.
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