A transgenic zebrafish model of neutrophilic inflammation

Stephen A. Renshaw(University of Sheffield), Catherine A. Loynes(University of Sheffield), Daniel M.I. Trushell(University of Sheffield), Stone Elworthy(University of Sheffield), Philip W. Ingham(University of Sheffield), Moira K. B. Whyte(University of Sheffield)
Blood
August 23, 2006
10.1182/blood-2006-05-024075
Cited by 1,033Open Access
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Abstract

We have established an in vivo model for genetic analysis of the inflammatory response by generating a transgenic zebrafish line that expresses GFP under the neutrophil-specific myeloperoxidase promoter. We show that inflammation is induced after transection of the tail of zebrafish larvae and that this inflammation subsequently resolves over a similar time course to mammalian systems. Quantitative data can be generated from this model by counting of fluorescent cells or by digital image analysis. In addition, we show that the resolution of experimentally induced inflammation can be inhibited by the addition of a pancaspase inhibitor, zVD.fmk, demonstrating that experimental manipulation of the resolution of inflammation is possible in this model.

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