A chromosome 9 deletion in Plasmodium falciparum results in loss of cytoadherence

Darrell J. Kemp(Walter and Eliza Hall Institute of Medical Research), Jay Daniel Thompson(Walter and Eliza Hall Institute of Medical Research), Debra A. Barnes(Walter and Eliza Hall Institute of Medical Research), Tony Triglia(Walter and Eliza Hall Institute of Medical Research), Freda Karamalis(Walter and Eliza Hall Institute of Medical Research), Carolyn Petersen(University of California, San Francisco), Graham V. Brown(Walter and Eliza Hall Institute of Medical Research), Karen P. Day(Imperial College London)
Memórias do Instituto Oswaldo Cruz
January 1, 1992
10.1590/s0074-02761992000700011
Cited by 29Open Access
Full Text

Abstract

Many lines of Plasmodium falciparum undergo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and the selected cells produce gametocytes. The deletion also results in loss of expression of PfEMP1, the putative cytoadherence ligand, suggesting that PfEMP1 or a regulatory gene controlling PfEMP1 expression and gametocytogenesis may be encoded in this region. We have isolated several markers for the deleted region and are currently using a YAC-P. falciparum library to investigate this region of the genome in detail.

Related Papers

No related papers found

Powered by citation graph analysis