Cloning and expression of the human erythropoietin gene.
F K Lin(Amgen (United States)), Sidney V. Suggs(Amgen (United States)), Chih-Wei Lin(Amgen (United States)), Jeffrey K. Browne(Amgen (United States)), Ralph Smalling(Amgen (United States)), Joan C. Egrie(Amgen (United States)), K K Chen(Amgen (United States)), Gary M. Fox(Amgen (United States)), Frank Martin(Amgen (United States)), Zippora Stabinsky(Amgen (United States))
Cited by 1,147Open Access
Abstract
The human erythropoietin gene has been isolated from a genomic phage library by using mixed 20-mer and 17-mer oligonucleotide probes. The entire coding region of the gene is contained in a 5.4-kilobase HindIII-BamHI fragment. The gene contains four intervening sequences (1562 base pairs) and five exons (582 base pairs). It encodes a 27-amino acid signal peptide and a 166-amino acid mature protein with a calculated Mr of 18,399. The erythropoietin gene, when introduced into Chinese hamster ovary cells, produces erythropoietin that is biologically active in vitro and in vivo.
Related Papers
No related papers found
Powered by citation graph analysis