Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line
Piotr Masiakowski(Centre National de la Recherche Scientifique), Richard Breathnach(Centre National de la Recherche Scientifique), Jacques Bloch(Centre National de la Recherche Scientifique), Frank Gannon(Inserm), A. Krust(Inserm), Pierre Chambon(Centre National de la Recherche Scientifique)
Cited by 727Open Access
Abstract
cDNA clones corresponding to a mRNA whose level is rapidly increased by addition of oestradiol to the culture medium have been isolated by differential screening of a cDNA library from the breast cancer cell line MCF-7, which contains oestrogen receptors. Such clones will be useful in studies of the DNA sequences required for hormonal induction and to determine whether expression of the corresponding gene is in any way related to the cancerous state. We have also obtained a cDNA clone for a messenger whose level is apparently decreased by steroid hormones.
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