Isolating and engineering human antibodies using yeast surface display

Ginger Chao(Massachusetts Institute of Technology), Wai Leung Lau(Massachusetts Institute of Technology), Benjamin J. Hackel(Massachusetts Institute of Technology), Stephen L. Sazinsky(Massachusetts Institute of Technology), Shaun M. Lippow(Massachusetts Institute of Technology), K. Dane Wittrup(Massachusetts Institute of Technology)
Nature Protocols
July 13, 2006
10.1038/nprot.2006.94
Cited by 949

Abstract

Related Papers

Phenotypic Analysis of Antigen-Specific T Lymphocytes
John D. Altman, Paul Moss, Philip Goulder et al.|Science|1996|3.6k
Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method
R. Daniel Gietz, Robin A. Woods|Methods in enzymology on CD-ROM/Methods in enzymology|2002|3k
Rapid evolution of a protein in vitro by DNA shuffling
Willem P.C. Stemmer|Nature|1994|1.9k
Yeast surface display for screening combinatorial polypeptide libraries
Eric T. Boder, K. Dane Wittrup|Nature Biotechnology|1997|1.8k
Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity
Eric T. Boder, Katarina S. Midelfort, K. Dane Wittrup|Proceedings of the National Academy of Sciences|2000|689