Whole-Genome Shotgun Sequencing of Mitochondria from Ancient Hair Shafts

M. Thomas P. Gilbert(University of Copenhagen), Lynn P. Tomsho(University of Copenhagen), Snjezana Rendulic(University of Copenhagen), Michael Packard(University of Copenhagen), Daniela I. Drautz(University of Copenhagen), Andrei Sher(University of Copenhagen), Alexei Tikhonov(University of Copenhagen), Love Dalén(University of Copenhagen), Т. В. Кузнецова(University of Copenhagen), П. А. Косинцев(University of Copenhagen), Paula F. Campos(University of Copenhagen), Thomas Higham(University of Copenhagen), Matthew J. Collins(University of Copenhagen), A. S. Wilson(University of Copenhagen), Fyodor Shidlovskiy(University of Copenhagen), Bernard Buigues(University of Copenhagen), Per G. P. Ericson(University of Copenhagen), Mietje Germonpré(University of Copenhagen), Anders Götherström(University of Copenhagen), Paola Iacumin(University of Copenhagen), В. И. Николаев(University of Copenhagen), Małgosia Nowak-Kemp(University of Copenhagen), Eske Willerslev(University of Copenhagen), James Knight(University of Copenhagen), Gerard P. Irzyk(University of Copenhagen), Clotilde Perbost(University of Copenhagen), Karin M. Fredrikson(University of Copenhagen), Timothy T. Harkins(University of Copenhagen), Sharon Sheridan(University of Copenhagen), Webb Miller(University of Copenhagen), Stephan C. Schuster(University of Copenhagen)
Science
September 27, 2007
10.1126/science.1146971
Cited by 274

Abstract

Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.

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