Cytoskeletal Regulation by the Nedd8 Ubiquitin-Like Protein Modification Pathway
Thimo Kurz(University of Oregon), Lionel Pintard(Swiss Cancer Center Léman), John H. Willis(University of Oregon), Danielle R. Hamill(University of Oregon), Pierre Gönczy(Swiss Cancer Center Léman), Matthias Peter(Swiss Cancer Center Léman), Bruce Bowerman(University of Oregon)
Cited by 197Open Access
Abstract
The Nedd8 ubiquitin-like protein modification pathway regulates cell-cycle progression. Our analysis of Nedd8 requirements during Caenorhabditis elegans embryogenesis indicates that the cytoskeleton is another target. Nedd8 conjugation negatively regulated contractility of the microfilament-rich cell cortex during pronuclear migration and again during cytokinesis. The Nedd8 pathway also was required after meiosis to negatively regulate katanin, a microtubule-severing complex, permitting the assembly of a large mitotic spindle. We propose that Nedd8-modified cullin, as part of an E3 ubiquitin ligase complex, targets katanin for degradation during the transition from meiosis to mitosis.