Identification of gene expression profile of dorsal root ganglion in the rat peripheral axotomy model of neuropathic pain

Huasheng Xiao(Chinese National Human Genome Center at Shanghai), Qiuhua Huang(Chinese National Human Genome Center at Shanghai), Fang‐Xiong Zhang(Chinese National Human Genome Center at Shanghai), Lan Bao(Chinese National Human Genome Center at Shanghai), Ying‐Jin Lu(Chinese National Human Genome Center at Shanghai), Chao Guo(Chinese National Human Genome Center at Shanghai), Liang Yang(Chinese National Human Genome Center at Shanghai), Wein-Jing Huang(Chinese National Human Genome Center at Shanghai), Gang Fu(Chinese National Human Genome Center at Shanghai), Shuhua Xu(Chinese National Human Genome Center at Shanghai), Xiping Cheng(Chinese National Human Genome Center at Shanghai), Qing Yan(Chinese National Human Genome Center at Shanghai), Zhidong Zhu(Chinese National Human Genome Center at Shanghai), Xin Zhang(Chinese National Human Genome Center at Shanghai), Zhu Chen(Chinese National Human Genome Center at Shanghai), Ze‐Guang Han(Chinese National Human Genome Center at Shanghai), Xu Zhang(Chinese National Human Genome Center at Shanghai)
Proceedings of the National Academy of Sciences
June 11, 2002
10.1073/pnas.122231899
Cited by 501

Abstract

Phenotypic modification of dorsal root ganglion (DRG) neurons represents an important mechanism underlying neuropathic pain. However, the nerve injury-induced molecular changes are not fully identified. To determine the molecular alterations in a broader way, we have carried out cDNA array on the genes mainly made from the cDNA libraries of lumbar DRGs of normal rats and of rats 14 days after peripheral axotomy. Of the 7,523 examined genes and expressed sequence tags (ESTs), the expression of 122 genes and 51 expressed sequence tags is strongly changed. These genes encompass a large number of members of distinct families, including neuropeptides, receptors, ion channels, signal transduction molecules, synaptic vesicle proteins, and others. Of particular interest is the up-regulation of gamma-aminobutyric acid(A) receptor alpha5 subunit, peripheral benzodiazepine receptor, nicotinic acetylcholine receptor alpha7 subunit, P2Y1 purinoceptor, Na(+) channel beta2 subunit, and L-type Ca(2+) channel alpha2delta-1 subunit. Our findings therefore reveal dynamic and complex changes in molecular diversity among DRG neurons after axotomy. Sequences reported in this paper have been deposited in the GenBank database (accession numbers BG 662484-BG 673712)

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