Ultrasensitive Stain for Proteins in Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid Proteins
Carl R. Merril(National Institute of Mental Health), David Goldman(National Institute of Mental Health), S A Sedman(National Institute of Mental Health), Michael H. Ebert(National Institute of Mental Health)
Cited by 2,947
Abstract
A new silver stain for electrophoretically separated polypeptides can be rapidly and easily used and can detect as little as 0.01 nanogram of protein per square millimeter. When employed with two-dimensional electrophoresis, it should permit qualitative and quantitative characterization of protein distributions in body fluids and tissues. It has been used to demonstrate regional variations in cerebrospinal fluid proteins.
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