Distinct Functions of SR Proteins in Alternative pre-mRNA Splicing

Alan M. Zahler(Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa), Karla M. Neugebauer(Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa), William S. Lane(Harvard University Press), Mark B. Roth(Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa)
Science
April 9, 1993
10.1126/science.8385799
Cited by 408

Abstract

Alternative splicing of precursor messenger RNAs (pre-mRNAs) is a common mechanism of regulating gene expression. SR proteins are a family of pre-mRNA splicing factors that are structurally related and evolutionarily conserved. Any member of the SR family can complement a splicing-deficient extract that lacks the entire family of SR proteins. Here it is demonstrated that particular SR proteins have distinct functions in alternative pre-mRNA splicing in vitro. In addition, SR proteins are differentially expressed in a variety of tissues. These results suggest a fundamental role for SR proteins in the regulation of alternative splicing.

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