Neutralization of the AIDS Retrovirus by Antibodies to a Recombinant Envelope Glycoprotein
Laurence A. Lasky(Sunesis (United States)), Jerome E. Groopman(Deaconess Hospital), C Fennie(Sunesis (United States)), Patricia Benz(Deaconess Hospital), Daniel J. Capon(Sunesis (United States)), Donald Dowbenko(Sunesis (United States)), Gerald Nakamura(Sunesis (United States)), Wylla M. Nunes(Sunesis (United States)), Mark Renz(Sunesis (United States)), Phillip W. Berman(Sunesis (United States))
Cited by 358
Abstract
Mammalian cell lines have been engineered to produce a secreted form of the AIDS retrovirus envelope glycoprotein. The recombinant protein has been isolated from growth-conditioned culture media and used to immunize animals. Antibodies directed against the recombinant molecule were found to react with the envelope glycoprotein produced in virus-infected cells. Furthermore, these antibodies were able to directly inactivate the AIDS retrovirus in a neutralization assay in vitro. The expression system reported here should provide sufficient quantities of the AIDS retrovirus envelope protein for biological and vaccination studies.
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