High Performance Liquid Cbromatographic Separation of Globin Chains on a Large-Pore C<sub>4</sub>Column

Joan B. Shelton(California Institute of Technology), J. Roger Shelton(California Institute of Technology), W. A. Schroeder(California Institute of Technology)
Journal of Liquid Chromatography
September 1, 1984
10.1080/01483918408068849
Cited by 420

Abstract

Abstract Excellent resolution of human and baboon globin chains may be obtained by HPLC on a Vydac large-pore C4 column. The procedure is rapid and uses a gradient between aqueous trifluoroacetic acid and trifluoroacetic acid in acetonitrile. The common human γ chains are easily separable from each other as are some α- and β-chain variants from the normal chains and from each other.

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